Open Access Highly Accessed Research article

Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: Pathway description and gene discovery for production of next-generation biofuels

Hamid Rismani-Yazdi12, Berat Z Haznedaroglu1, Kyle Bibby1 and Jordan Peccia1*

Author Affiliations

1 Department of Chemical and Environmental Engineering, Yale University, New Haven, CT 06511, USA

2 Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

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BMC Genomics 2011, 12:148  doi:10.1186/1471-2164-12-148

Published: 14 March 2011

Additional files

Additional file 1:

Comparison of D. tertiolecta transcriptome assembly outputs obtained using Newbler v2.3 and 2.5.

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Additional file 2:

A phylogenetic tree inferring the evolutionary relationship between D. tertiolecta and model microalgae Volvox carteri, and Chlamydomonas reinhardtii. The tree was generated using 18 S rRNA gene sequences of D. tertiolecta, Volvox carteri, and Chlamydomonas reinhardtii extracted from the NCBI database. Sequences were aligned using ClustalX and bootstrapping was performed in ClustalX with 100 iterations and values were displayed on the branch edges. The tree was visualized and published in Mega5. The distance bar represents 0.01 base changes/base. The tree was rooted with Methanobacterium congolense (NCBI Accession Number: AF233586.1).

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Additional file 3:

Candidate genes identified based on KEGG orthology (KO) annotation of the D. tertiolecta transcriptome.

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Additional file 4:

β-oxidation pathway of D. tertiolecta based on the annotation of transcriptome and KEGG pathway assignment.

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Additional file 5:

Glycolysis pathway of D. tertiolecta based on the annotation of transcriptome and KEGG pathway assignment.

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Additional file 6:

Enzymes involved in glycolysis identified by annotation of the D. tertiolecta transcriptome.

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