Transcription profile of bovine mucins in the gastrointestinal tract. Transcription profile of membrane associated and secreted mucins in the gastro-intestinal tract. PCR on cDNA samples was used to characterize the transcription profile of membrane associated and secreted mucins in twelve regions of the gastro-intestinal tract. Squares represent PCR products of individual animals (n = 3 in oesophagus; n = 4 in all other tissues). Presence and intensity of gel bands were analyzed using ImageQuant™350 and are reported as absent (green), present weak (yellow), and present strong (red). Glyceraldehyde 3-phosphate (GAPDH) and β-actin (ACTB) were used to control the quality of the synthesized cDNA in all the samples.
Hoorens et al. BMC Genomics 2011 12:140 doi:10.1186/1471-2164-12-140