Open Access Highly Accessed Research article

Advancing Eucalyptus genomics: identification and sequencing of lignin biosynthesis genes from deep-coverage BAC libraries

Jorge AP Paiva12*, Elisa Prat3, Sonia Vautrin3, Mauro D Santos4, Hélène San-Clemente56, Sérgio Brommonschenkel7, Paulo GS Fonseca4, Dario Grattapaglia8, Xiang Song9, Jetty SS Ammiraju9, David Kudrna9, Rod A Wing9, Ana T Freitas4, Hélène Bergès3 and Jacqueline Grima-Pettenati56

Author Affiliations

1 Instituto de Investigação Científica Tropical (IICT), Centro de Florestas e dos Produtos Florestais, Tapada da Ajuda, 1349-018 Lisboa, Portugal

2 Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal

3 INRA-CNRGV, Chemin de Borde Rouge, 31326 Castanet-Tolosan, France

4 Instituto de Engenharia de Sistemas e Computadores: Investigação e Desenvolvimento (INESC-ID/IST), R. Alves Redol 9, 1000-029 Lisboa, Portugal

5 Université de Toulouse; UPS; UMR 5546, Surfaces Cellulaires et Signalisation chez les Végétaux; BP 42617, F-31326, Castanet-Tolosan, France

6 CNRS; UMR 5546; BP 42617, F-31326, Castanet-Tolosan, France

7 BIOAGRO - Federal University of Viçosa, Av. P. H. Rolfs, s/n - 36570-000 - Viçosa, MG, Brasil

8 EMBRAPA Genetic Resources and Biotechnology, EPqB Final W5 NOrte, 70770-910 Brasilia, DF, Brazil

9 Arizona Genomics Institute, School of Plant Sciences and BIO5 Institute, The University of Arizona, Tucson AZ 85721, USA

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BMC Genomics 2011, 12:137  doi:10.1186/1471-2164-12-137

Published: 4 March 2011

Additional files

Additional file 1:

BAC sequences characteristics.

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Additional file 2:

Analysis of polymorphism found at promoter of CCR gene and intron of CAD gene beetween the E. grandis (Transversaria section) and E. gunnii (Maidenaria section).

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Additional file 3:

Screening of the E. grandis BAC libraries for chloroplast and mitochondria genomes. Primers used for PCR amplification of organelles specific probes.

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Additional file 4:

Table of primers used to validate hybridization positive clones.

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Additional file 5:

E. grandis chloroplast genome annotation pipeline.

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