A decrease in ELK1 expression leads to elimination of the regulatory effect of HNF4α knock-down on a subset of genes. HepG2 cells were treated with non-specific siRNA control (siControl), HNF4α shRNA plasmid (siHNF4), siELK1 or both siHNF4 and siELK1. The expressions of COL4A1 (collagen, type IV, alpha 1), ZNF175 (zinc finger protein 175), MMP15 (matrix metallopeptidase 15) and SEC24A (SEC24 family, member A) genes were determined by real-time PCR. The abundance of mRNA in the siControl was set at 1. Data are presented as mean ± SD of three different experiments. *p < 0.05 and **p < 0.01 indicate a significant difference compared to siControl. #p < 0.01 indicates a significant difference compared to siHNF4α.
Wang et al. BMC Genomics 2011 12:128 doi:10.1186/1471-2164-12-128