The ELK1 expression in HepG2 cells with HNF4α or ELK1 knock-down. HepG2 cells were transfected with non-specific siRNA control (siControl), HNF4α shRNA plasmid (siHNF4) or siELK1, and then treated with or without cytokines. The expression of ELK1 was measured by real-time PCR. The results represent the relative mRNA expression level normalized to GAPDH mRNA level. The abundance of mRNA in the controls was set at 1. Data represent mean ± SD of three different experiments. *p < 0.05 and **p < 0.01 indicate a significant difference compared to siControls.
Wang et al. BMC Genomics 2011 12:128 doi:10.1186/1471-2164-12-128