Table 3

Real time RT-PCR expression analysis of selected melon genes.

ID

Homology

E-value

Annotation

ISPaVe strain/dpi

I/R

Fold-change (Infect. vs. mock)

SD


P200

MU48195

0

ACC oxidase

1070/4 dpi

I

7.04

2.80

P557

MU51460

3E-45

Respiratory burst oxidase homolog protein C

1070/4 dpi

I

61.92

14.73

P1100

MU54228

2E-44

Protein translocase SEC61

1018/2 dpi

R

1.87

0.37

P1370

MU50486

1E-112

calcium-dependent protein kinase CDPK1444

1070/4 dpi

I

3.81

0.66

P554

MU45886

2E-27

13S-lipoxygenase

1018/21 dpi

I

1.26

0.14

P767

MU47701

4E-50

Endochitinase 1 precursor

1018/21 dpi

I

473.05

146.45

P769

MU43951

6E-61

UBQ14 (ubiquitin 14)

1018/21 dpi

I

25.34

24.29


Seven melon transcripts, differentially expressed following Fusarium oxysporum f. sp. melonis (FOM) infection according to cDNA-AFLP experiments, were selected for validation by real time RT-PCR. Each gene was validated at the same time point and with the same FOM strain that generated a differential cDNA-AFLP profile. The FOM strains are ISPaVe170 race1 and ISPaVe1018 race 1,2. Results are shown as fold change values in infected samples in comparison to mock-inoculated controls and are the average of three technical replicates. I/R: induced or repressed according to cDNA-AFLP experiments. SD: standard deviation.

Sestili et al. BMC Genomics 2011 12:122   doi:10.1186/1471-2164-12-122

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