Precision of real-time PCR platforms. Within-run qPCR precision across a range of transcript abundance levels (copies per ng total RNA) are displayed for cDNA or pre-amplified cDNA quantified using ABI 7900HT system or BioMark. Mean variation (SD) of Ct values ± SD is displayed based on data from eight different RNA standards from three independent qPCR experiments.
Devonshire et al. BMC Genomics 2011 12:118 doi:10.1186/1471-2164-12-118