Linear range of RT-qPCR platforms. Example plot showing detection of an ERCC RNA standard (ERCC-42) across a range of transcript copy numbers from 1 to 106 copies per ng total RNA with cDNA (triangles) or pre-amplified cDNA (diamonds) as the template on (A) BioMark (B) ABI 7900HT system. Data-points are displayed as individual qPCR replicates. Dotted line indicates linear detection range.
Devonshire et al. BMC Genomics 2011 12:118 doi:10.1186/1471-2164-12-118