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Open Access Methodology article

Applicability of RNA standards for evaluating RT-qPCR assays and platforms

Alison S Devonshire, Ramnath Elaswarapu* and Carole A Foy

Author Affiliations

LGC Limited, Queens Road, Teddington, Middlesex, TW11 0LY, UK

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BMC Genomics 2011, 12:118  doi:10.1186/1471-2164-12-118

Published: 18 February 2011

Abstract

The availability of diverse RT-qPCR assay formats and technologies hinder comparability of data between platforms. Reference standards to facilitate platform evaluation and comparability are needed. We have explored using universal RNA standards for comparing the performance of a novel qPCR platform (Fluidigm® BioMark™) against the widely used ABI 7900HT system. Our results show that such standards may form part of a toolkit to evaluate the key performance characteristics of platforms.