Global expression changes. 293T cells were reverse-transfected with inducible constructs, each arrayed over 75 replicate spots, and fluorescent cells were imaged over 24 hours. Single-cell measurements at each spot were averaged for the Venus (orange) and ECFP (blue) channels and are shown over time for (A) p(GRE)x3-AdMLP-VC5 following induction with 1 × 10-7M dexamethasone, (B) pTetRE-VC5 following induction with 2.2 × 10-6M doxycycline, (C) p(RARE)x3-AdMLP-VC5 following induction with 1 × 10-6M all-trans retinoic acid and (D) the promoterless VC5 reporter. The mean of all replicate fields is drawn in red for Venus signal and black for ECFP signal.
Rajan et al. BMC Genomics 2011 12:115 doi:10.1186/1471-2164-12-115