Table 3

Difference in mRNA levels between Fat (F) and Lean (L) and between G+ and G- muscles for genes chosen for quantification by real-time RT-PCR

Symbol

Name

G+/G-

F/L


Microarray

qRT-PCR

Microarray

qRT-PCR


Genes screened out from the microarray analyses

ABHD5

Abhydrolase domain containing 5

1.367*

0.997

NS

1.527*

ACSL1

Acyl-CoA synthetase long-chain family member 1

1.391*

0.994

NS

0.765

CEBPB

CCAAT/enhancer binding protein (C/EBP), beta

1.491*

3.266**

NS

0.828

ETFA

Electron-transfer-flavoprotein, alpha polypeptide

0.719*

1.036

0.896*

0.896

FOXO3

Forkhead box O3

1.329*

1.400

NS

1.146

LPAR1

Lysophosphatidic acid receptor 1

0.511*

0.472*

NS

1.243

PDK4

Pyruvate dehydrogenase kinase, isozyme 4

0.645*

0.397†

ND

3.007†

PIK3CD

Phosphoinositide-3-kinase, catalytic, delta polypeptide

1.768*

0.856

NS

1.613

PPP1R12B

Protein phosphatase 1, regulatory (inhibitor) subunit 12B

0.758*

0.776

NS

1.103

RGS2

Regulator of G-protein signaling 2

1.778*

2.433*

1.454*

0.821

RPIA

Ribose 5-phosphate isomerase A

1.380*

0.945

NS

1.352

RPS6

Ribosomal protein S6

0.611*

0.678†

NS

1.014

SRF

Serum response factor (c-fos serum response element-binding transcription factor)

0.562*

0.405*

0.734*

0.734

UCP3

Uncoupling protein 3 (mitochondrial, proton carrier)

0.641*

0.601

NS

0.731

UGDH

UDP-glucose dehydrogenase

NS

0.503†

1.441*

1.477

UGP2

UDP-glucose pyrophosphorylase 2

1.469*

0.873

NS

0.898


Candidate genes chosen for targeted analyses by real-time RT-PCR§

PRKAB1

AMP-activated, beta 1 non-catalytic subunit

ND

0.603†

ND

1.315

PRKAG2

AMP-activated, gamma 2 non-catalytic subunit

ND

0.517*

ND

1.129

PHKD

Phosphorylase kinase, delta

ND

0.683*

ND

1.115


For both microarray and real-time RT-PCR analyses, results are expressed as Fat to Lean and G+ to G- ratios of the expression gene.

NS = non-significant; ND = not determined. Statistical significance is indicated as follows: †p value < 0.1, *p value < 0.05, **p value < 0.01.

§ Only genes identified as differentially expressed in at least one model are presented in the Table.

Sibut et al. BMC Genomics 2011 12:112   doi:10.1186/1471-2164-12-112

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