Error rate of sequencing reactions. Box and whisker plots show median, 25-75 percentile, and range for erroneous sequences for the 5C.C7 (A), OT-1 (B), and DO11.10 (C) CDR3 sequences expressed as a percent of total sequence events that met initial criteria. Phred values were used to further constrain sequence sets and the minimal phred cutoff score for any nt in a sequence is indicated.
Nguyen et al. BMC Genomics 2011 12:106 doi:10.1186/1471-2164-12-106