Table 5

RT-PCR validation.

Library Origin

Selected ASSETs

ASSET validation

Heteroduplex validation

Cross-validation


Lib_1

6

5

2

4

Lib_2

7

7

4

7

Lib_1 & Lib_2

5

5

0

-


Library Origin: library where the ASSET was captured from; selected ASSETs: number of randomly selected ASSETs; ASSET validation: to confirm the presence of the ASSET in the same RNA used for library construction; heteroduplex validation: amplification of alternatively spliced transcripts that could have participated in the heteroduplex formation; and cross-validation: amplification of ASSETs specifically captured from one library using the cDNA template from the other library.

Ferreira et al. BMC Genomics 2010 11(Suppl 5):S4   doi:10.1186/1471-2164-11-S5-S4

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