Figure 6.

The influence of ERBB2-mediated expression on the regulation of AS variants. In the left panel, a schema of the microfluidic capillary electrophoresis approach is shown. The exons are represented by numbered squares according to the exons involved in the AS events for each gene. The black arrows represent the primers used for PCR amplification (PF - forward primer; PR - reverse primer). The electropherogram represents the amplification of the AS variants for the Hb4a cell line (blue line) and for the C5.2 cell line (red line). The green arrows indicates internal markers: LM (lower marker) and UM (upper marker). In the right panel, the probe-ligation approach is shown. Each pair of probe is shown for each AS variant separately, with the corresponding PCR products on 8% acrylamide gel. M - 100 bp ladder. A - gene, B - FLNA and C -TRIP6.