siRNA-induced knowndown of CD49e in cultured human thymic epithelial down-regulates other genes related to cell adhesion. The expression of neuropilin-2 (Nrp2), vascular endothelial growth factor (VEGF) and plexin A mRNA are seen in the upper panels, whereas neuronal cell adhesion molecule (NrCAM), and phosphatidylinositol-4-phosphate 5-kinase (PIP5K) are seen in bottom panels. In all cases, real-time quantitative PCR was applied to compare CD49e-siRNA (black bars) with scramble-siRNA (open bars) treated human TEC. Analyses were carried out 48 hours post-transfection. Results were normalized to β-glucuronidase mRNA and GAPDH mRNA and they are expressed relative to the mRNA levels in CD49e-siRNA versus scramble-siRNA in transfected cells; results are representative of three independent experiments performed in triplicate. Bars the mean values ± SE; and the data were validated using Mann-Whitney test (p ≤ 0.05).
Linhares-Lacerda et al. BMC Genomics 2010 11(Suppl 5):S2 doi:10.1186/1471-2164-11-S5-S2