This article is part of the supplement: Proceedings of the 5th International Conference of the Brazilian Association for Bioinformatics and Computational Biology (X-meeting 2009)

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RNA interference-mediated knockdown of CD49e (α5 integrin chain) in human thymic epithelial cells modulates the expression of multiple genes and decreases thymocyte adhesion

Leandra Linhares-Lacerda1, Marcelo Ribeiro-Alves2, Ana Cristina Martins de Almeida Nogueira3, Daniella Areas Mendes-da-Cruz1, Danielle Aparecida Magalhães5, Mireille Dardenne4, Geraldo Aleixo Passos5 and Wilson Savino1*

Author Affiliations

1 Laboratory on Thymus Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 21040-360, Brazil

2 Center for Technology Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 21040-360, Brazil

3 Department of Immunology, National Institute of Quality Control in Health, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 21040-360, Brazil

4 Hôpital Necker, Université Paris Descartes, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8147, Paris, 75743, France

5 Molecular Immunogenetics Group, Department of Genetics, University of São Paulo School of Medicine, Ribeirão Preto, SP, 14049-900, Brazil

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BMC Genomics 2010, 11(Suppl 5):S2  doi:10.1186/1471-2164-11-S5-S2

Published: 22 December 2010

Additional files

Additional file 1:

Table I: Classification of the down- and up-regulated genes in siRNA-triggered CD49e silenced thymic epithelial cells. List of genes differentially express with annotation.

Format: XLS Size: 27KB Download file

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Additional file 2:

Tabel II: Down and upregulated genes in siRNA-triggered CD49e silenced thymic epithelial cells without annotation. List of genes differentially express without annotation.

Format: XLS Size: 16KB Download file

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Additional file 3:

Table III: Oligonucleotide sequences specifically tailored for used in real time PCR. GeneBank information and oligonucleotide sequences used in real time PCR.

Format: XLS Size: 14KB Download file

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