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This article is part of the supplement: Ninth International Conference on Bioinformatics (InCoB2010): Computational Biology

Open Access Proceedings

Construction of a genetic AND gate under a new standard for assembly of genetic parts

Shotaro Ayukawa1, Akio Kobayashi1, Yusaku Nakashima2, Hidemasa Takagi2, Shogo Hamada1, Masahiko Uchiyama1, Katsuyuki Yugi26, Satoshi Murata3, Yasubumi Sakakibara2, Masami Hagiya4, Masayuki Yamamura1 and Daisuke Kiga15*

Author Affiliations

1 Department of Computational Intelligence and Systems Science, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8503, Japan

2 Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan

3 Department of Bioengineering and Robotics, Tohoku University, 6-6-01 Aoba-yama, Sendai 980-8579, Japan

4 Department of Computer Science, Graduate School of Information Science and Technology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

5 PRESTO, JST, 4-1-8 Honcho, Kawaguchi-shi, Saitama 332-0012, Japan

6 Present address: Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

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BMC Genomics 2010, 11(Suppl 4):S16  doi:10.1186/1471-2164-11-S4-S16

Published: 2 December 2010

Abstract

Background

Appropriate regulation of respective gene expressions is a bottleneck for the realization of artificial biological systems inside living cells. The modification of several promoter sequences is required to achieve appropriate regulation of the systems. However, a time-consuming process is required for the insertion of an operator, a binding site of a protein for gene expression, to the gene regulatory region of a plasmid. Thus, a standardized method for integrating operator sequences to the regulatory region of a plasmid is required.

Results

We developed a standardized method for integrating operator sequences to the regulatory region of a plasmid and constructed a synthetic promoter that functions as a genetic AND gate. By standardizing the regulatory region of a plasmid and the operator parts, we established a platform for modular assembly of the operator parts. Moreover, by assembling two different operator parts on the regulatory region, we constructed a regulatory device with an AND gate function.

Conclusions

We implemented a new standard to assemble operator parts for construction of functional genetic logic gates. The logic gates at the molecular scale have important implications for reprogramming cellular behavior.