Visualization of bias of popular DEG identification algorithms. FC has many false positive predictions for genes with low average expressions or small expression differences. RP’s false positives are sparsely located in low expression and small average difference region. tTest’s false positives are dominated by genes with low average difference. WAD has less false positives than other algorithms.
Hu and Xu BMC Genomics 2010 11(Suppl 2):S3 doi:10.1186/1471-2164-11-S2-S3