Comparison of the normalization factors calculated using different reference gene(s). Normalization factors (NFs) calculated with RPL19/RPL29, ACTB and GAPDH were compared to that calculated by the top 2 reference genes (NFtop2) as recommended by both NormFinder and geNorm, for each stimulus. The percentage deviations of NFRPL19/RPL29; NFACTB; NFGAPDH from NFtop2 (|NFx-NFtop2|/NFtop2) were represented by box plot. The 25th percentile to the 75th percentile (boxes), and ranges (whiskers) were shown.
Zhou et al. BMC Genomics 2010 11:75 doi:10.1186/1471-2164-11-75