Tiling approach to Southern blot probe design. Many potential probes are generated and tested in the user-specified genomic window, starting from the maximum allowable probe length (default 1300), tiling the window by moving by a small percentage of the probe length each time (default 5%). Once this is completed the probe length is reduced by 50 bases (configurable) and the window re-tiled generating more candidate probes. The process is repeated until the minimum probe length is reached (default 500).
Croning et al. BMC Genomics 2010 11:74 doi:10.1186/1471-2164-11-74