Additional file 4.

Accuracy of gene structure prediction for AP2/ERF genes in the Grapevine Genome Browser (X8). (1A) Gene structure of four V. vinifera ERF sequences taken as examples provided by the genoscope database. GSVIVT00034010001, whose structure is supported by cDNA sequencing (Grape Genome Browser [X8]), GSVIVT000009519001, GSVIVT0009456001, GSVIVT00021812001. Exons are depicted as empty squares and introns as single lines, with their size displayed at the top. Positions of the primers used for PCR amplification are indicated by arrows. Forward and reverse primers marked as "a" were used to test the presence of the complete sequence of the predicted cDNA. Primers marked with (b) were used in combination with the corresponding forward or reverse "a" primers to test the presence of the same cDNA. (1B) PCR-products obtained using the "a" and "a+b" primers with stem tissue cDNA as template.

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Licausi et al. BMC Genomics 2010 11:719   doi:10.1186/1471-2164-11-719