Figure 4.

Splice scores compare well with RT-PCR data. Tissue-specific alternative splicing data are shown for four genes: FEZ2 (A), TPD52 (B), CAMK2D (C) and MINK1 (D). In each panel schematic representations of the analyzed splice junctions are shown on top. Exons are depicted as boxes and numbered; lines connecting the exons specify exon junctions; arrows show the position of PCR primers used for the RT-PCR gel electrophoresis splicing analysis. Below the schematics are graphs that plot splice scores for three sets of data: sequenced asMIPs (squares), array quantified asMIPs (diamonds) and qPCR (triangles). The M-score data are plotted for five different tissues: placenta (PLA), skeletal muscle (SKM), stomach (STM), cerebellum (CEB) and frontal lobe (FRL). Analyzed exon junctions are color-coded and specified to the right of each graph (e.g. "5-6" corresponds to data for the junction between exons five and six). Gray dashed lines show the M-score cutoffs for qPCR (+/-2.7) and asMIP data (+/-1.3). Tissue-specific RT-PCR, gel electrophoresis data is pictured below each splice-score graph. PCR primers surrounding the analyzed exon junctions were used to amplify cDNA reverse transcribed from RNA extracted from the specified tissues. A reference sample (REF) containing reverse-transcribed total RNA from >20 human tissues is pictured to provide a pictorial approximation of baseline splicing for comparison with the graph. The exons contained within each PCR gel band are specified to the right of each gel.

Lin et al. BMC Genomics 2010 11:712   doi:10.1186/1471-2164-11-712
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