Open Access Highly Accessed Methodology article

Decoding pooled RNAi screens by means of barcode tiling arrays

Michael Boettcher1*, Johannes Fredebohm1, Amin Moghaddas Gholami1, Yafit Hachmo2, Iris Dotan2, Dan Canaani2 and Jörg D Hoheisel1

Author Affiliations

1 Department for Functional Genome Analysis, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany

2 Department of Biochemistry, Tel-Aviv University, 69978 Tel-Aviv, Israel

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BMC Genomics 2010, 11:7  doi:10.1186/1471-2164-11-7

Published: 5 January 2010

Additional files

Additional file 1:

Results from negative selection screen, Shown are the clone IDs as well as stem sequences and target genes from all 305 shRNA expression constructs included in the screen.

Format: XLS Size: 118KB Download file

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Additional file 2:

Results from negative selection screen, From the 278 constructs analyzable by the described conditions, (tend/tzero) log2 ratios are summarized from individual tiling probes as well as mean log2 ratios and p-values for each shRNA expression construct.

Format: XLS Size: 204KB Download file

This file can be viewed with: Microsoft Excel Viewer

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Additional file 3:

Description: Array data deposition information, Shown are ArrayExpress accession numbers.

Format: XLS Size: 27KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data