Detection of mRNAs associated with a Dcp2p-mCh immunoprecipitated protein fraction. Dcp2p-mCh, a protein component of P-bodies, was immunoprecipitated from whole cell protein extracts of wild type (wt) and myo1Δ strains. Total RNA was extracted from the immunoprecipitated fractions and RT-PCR analysis of mRNAs for RPS8A, RPL7B, RPL3 (left panels) and ACT1, CHS4, PIR3 (right panels) was performed. Mock immunoprecipitations were performed as a negative control for each experiment using total protein extract incubated with Protein A immunobeads alone followed by RT-PCR amplification of the eluted fraction.
Rivera-Ruiz et al. BMC Genomics 2010 11:690 doi:10.1186/1471-2164-11-690