Genome-wide binding of the orphan nuclear receptor TR4 suggests its general role in fundamental biological processes
- Equal contributors
1 Genome Center, University of California at Davis, Davis, CA 95616, USA
2 Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
3 Center for Computational Medicine and Bioinformatics, University of Michigan Medical School, Ann Arbor, MI 48109, USA
BMC Genomics 2010, 11:689 doi:10.1186/1471-2164-11-689Published: 2 December 2010
Additional file 1:
Validation of TR4 expression in four different cell types. Western blot analysis to validate expression of TR4 protein. 10 μg nuclear extract were loaded per lane. Cell types used are indicated above each lane.
Format: EPS Size: 749KB Download file
Additional file 2:
Primer sequences for standard and qPCR validation of TR4 ChIP samples.
Format: XLS Size: 28KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 3:
PCR anaslysis of three TR4 binding sites. ChIP assays were performed in K562 and HepG2 cells using TR4 antibody. PCR was performed using primers to TR4 binding sites identified by ChIP-seq (see Additional file 2 for oligo sequences). The enrichment of TR4 is shown in comparison to 0.1% Input chromatin. IgG ChIPs were used as negative controls. PCR analysis confirmed presence of TR4 at TNFIAP1, ECSIT and SCAP, but showed no significant enrichment when using negative control primers to ZNF333.
Format: EPS Size: 463KB Download file