Enhanced workflow for the detection of genes that are affected by differential splicing. A new definition of core set probe sets is the basis of the improved workflow. All probe intensities are summarised to exon expression levels. Estimation of detection above background noise leads to exon present calls. Exons that are absent in both sample groups will be removed by the background filter. In the statistical analysis, only genes with at least five present probe sets are considered. Both significance as well as magnitude of differential splicing are derived from an ANOVA based on a mixed linear model.
Langer et al. BMC Genomics 2010 11:676 doi:10.1186/1471-2164-11-676