Y2H array for detecting interactions with PDZ domains. An array of yeast strains each expressing one of the 93 C. elegans PDZ domains, together with a marker permitting growth on medium lacking leucine, and containing a second vector allowing growth on medium lacking tryptophan as well as expressing NRX-1 (left-hand panels) and PRY-1 (right-hand panels), spotted onto solid agar medium. The upper row shows that all strains grow on medium lacking leucine and tryptophan indicating that all strains contain both prey and bait vectors. In the lower row, on fully selective medium, lacking leucine, tryptophan and histidine, growth only occurs when there is an interaction between a PDZ domain and the protein of interest.
Lenfant et al. BMC Genomics 2010 11:671 doi:10.1186/1471-2164-11-671