Promoter activity of the mouse CRELD2-ALG12 gene pair in Neuro2a cells. Neuro2a cells were transfected with various luciferase reporter constructs containing successive 5'-deletion mutations of the mouse CRELD2 and ALG12 promoter sequences. A) Thirty-six hours after transfection, luciferase activity was measured. B) Twenty-four hours after transfection, the cells were incubated with or without Tg (0.1 μM) for 10 h, and luciferase activity was measured. The promoter activity in the absence (-) or presence (+) of Tg is shown as open and closed columns, respectively. Values represent means ± SD from 3 independent cultures and are expressed relative to the basal activity of the pGL3-Basic vector.
Oh-hashi et al. BMC Genomics 2010 11:664 doi:10.1186/1471-2164-11-664