Table 3

Composition of 'normal' and 'disease' panels

RNA standards

Copies/ng total RNA

Fold change (B/A)


Panel A

Panel B


13

1 × 105

1 × 105

1.0


42

1 × 104

5 × 103

0.5


81

1 × 102

1 × 102

1.0


84

1 × 102

5 × 102

5.0


95

1 × 103

1 × 103

1.0


99

8 × 103

1.2 × 104

1.5


113

1 × 101

1 × 101

1.0


171

1 × 101

1 × 102

10.0


To mimic the composition of 'normal' and 'disease' states which contain both differentially expressed genes (DEGs) and non-DEGs, two panels were produced using the eight standards at defined ratios and copy numbers.

Devonshire et al. BMC Genomics 2010 11:662   doi:10.1186/1471-2164-11-662

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