Technical reproducibility of microarray and RT-qPCR platforms. Technical reproducibility of microarray and RT-qPCR data was calculated based on the technical variation of each standard across three independent experiments using the same labelled sample (microarrays) or cDNA sample (RT-qPCR). Box-whisker plots show the median signal output (central line), interquartile range and 10th and 90th percentile values for each level of transcript abundance. Distribution of technical variation across all tested standards are displayed as percentage CV of microarray raw signal (SD/mean)(A), percentage variation associated with normalized signal intensity (log2) based on SD of normalized values (B), percentage variation in expression quantities (based on SD of Ct values, corrected for PCR efficiency of each assay)(C) and percentage variation in expression quantities, expressed relative to the mean Ct value for 106 copies/ng within each run (D).
Devonshire et al. BMC Genomics 2010 11:662 doi:10.1186/1471-2164-11-662