LOD of ERCC standards on microarray and RT-qPCR platforms. Percentage of assays exceeding the LOD for microarray (MA) and RT-qPCR platforms are displayed for each RNA standard for 1 copy/ng and 10 copies/ng total RNA abundance levels (Table 1), based on three replicate assays (microarrays) or nine replicate PCR assays (RT-qPCR). LOD is defined as the upper limit of the 95% confidence interval of 0 copies (microarrays) or positive Ct values (RT-qPCR).
Devonshire et al. BMC Genomics 2010 11:662 doi:10.1186/1471-2164-11-662