Open Access Highly Accessed Research article

Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

Caroline Joubert1, David Piquemal2, Benjamin Marie3, Laurent Manchon2, Fabien Pierrat2, Isabelle Zanella-Cléon4, Nathalie Cochennec-Laureau5, Yannick Gueguen1* and Caroline Montagnani1

Author Affiliations

1 Ifremer - Laboratoire de Biotechnologie et de Qualité de la Perle, Centre Océanologique du Pacifique - BP 7004, 98719 Taravao, French Polynesia

2 Skuldtech, 134, rue du Curat - Bat. Amarante, 34090 Montpellier, France

3 UMR CNRS 5561 Biogéosciences, Université de Bourgogne, 6, bd Gabriel, 21000 Dijon, France

4 IFR 128 BioSciences Gerland-Lyon Sud; UMR 5086 CNRS; IBCP, Institut de Biologie et Chimie des Protéines, Université de Lyon 1, 7 passage du Vercors, Lyon, F-69367, France

5 Ifremer, Centre de Nantes- rue de l'Ile d'Yeu - BP 21105, 44311 Nantes cedex 03, France

For all author emails, please log on.

BMC Genomics 2010, 11:613  doi:10.1186/1471-2164-11-613

Published: 1 November 2010

Abstract

Background

The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell.

Results

We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera biomineralization-related mantle protein sequences. Components of the current "chitin-silk fibroin gel-acidic macromolecule" model of biomineralization processes were found, in particular a homolog of a biomineralization protein (Pif-177) recently discovered in P. fucata. Among these sequences, we could show the localization of two other biomineralization protein transcripts, pmarg-aspein and pmarg-pearlin, in two distinct areas of the outer mantle epithelium, suggesting their implication in calcite and aragonite formation. Finally, by combining the EST approach with a proteomic mass spectrometry analysis of proteins isolated from the P. margaritifera shell organic matrix, we demonstrated the presence of 30 sequences containing almost all of the shell proteins that have been previously described from shell matrix protein analyses of the Pinctada genus. The integration of these two methods allowed the global composition of biomineralizing tissue and calcified structures to be examined in tandem for the first time.

Conclusions

This EST study made on the calcifying tissue of P. margaritifera is the first description of pyrosequencing on a pearl-producing bivalve species. Our results provide direct evidence that our EST data set covers most of the diversity of the matrix protein of P. margaritifera shell, but also that the mantle transcripts encode proteins present in P. margaritifera shell, hence demonstrating their implication in shell formation. Combining transcriptomic and proteomic approaches is therefore a powerful way to identify proteins involved in biomineralization. Data generated in this study supply the most comprehensive list of biomineralization-related sequences presently available among protostomian species, and represent a major breakthrough in the field of molluskan biomineralization.