Additional file 6.

Comparison of ORF codon usage for contigs assigned as either Varroa destructor or bacterial in origin. Scatterplot comparing G+C content and codon usage of ORFs from contigs assigned as either Varroa destructor or bacterial. The X-axis value is third position G+C and the Y-axis value is the codon usage statistic B [62], which as used here is a measure of the difference in codon usage between each subgroup of ORFs relative to the whole. The possible range of values for B is 0 to 2, with larger values corresponding to greater divergence in codon frequencies. A. Residual contigs after filtering, which are assumed to all derive from V. destructor. B. Contigs filtered because they have a higher BLAST score to bacterial sequences than to eukaryotic sequences. The two groups of contigs have distinct patterns of codon usage and nucleotide composition.

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Cornman et al. BMC Genomics 2010 11:602   doi:10.1186/1471-2164-11-602