Open Access Research article

Development and validation of a flax (Linum usitatissimum L.) gene expression oligo microarray

Stéphane Fenart1, Yves-Placide Assoumou Ndong2, Jorge Duarte3, Nathalie Rivière3, Jeroen Wilmer4, Olivier van Wuytswinkel2, Anca Lucau1, Emmanuelle Cariou5, Godfrey Neutelings1, Laurent Gutierrez6, Brigitte Chabbert7, Xavier Guillot8, Reynald Tavernier9, Simon Hawkins1 and Brigitte Thomasset10*

Author Affiliations

1 Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex, France

2 EA 3900-BioPI, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex, France

3 BIOGEMMA, Z.I. du Brezet, 8 rue des Frères Lumières, 63028 Clermont-Ferrand cedex 2, France

4 BIOGEMMA, domaine de Sandreau, Chemin de Panedautes, 31700 Mondonville, France

5 Institut Technique de Lin, 27170 Ecardenville La Campagne, France

6 CRRBM, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex, France

7 UMR- INRA, UMR614, URCA, FARE, 2 Esplanade R. Garros, CREA, BP 224, 51686 Reims, France

8 Laboulet Semences, 80 270 Airaines, France

9 LINEA, 20 Avenue Saget, 60 210 Grandvilliers, France

10 UMR CNRS 6022, GEC, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne cedex, France

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BMC Genomics 2010, 11:592  doi:10.1186/1471-2164-11-592

Published: 21 October 2010

Additional files

Additional file 1:

Analyses of differential gene expression between stem inner tissues and stem outer tissues sampled at vegetative and green capsule stages. Lists of differentially expressed genes and GO analyses (biological processes) for i) stem inner vegetative (SIV) vs stem outer vegetative (SOV), ii) stem inner green capsule (SIGC) vs stem outer green capsule (SOGC), iii) stem inner vegetative (SIV) vs stem inner green capsule (SIGC), iv) stem outer vegetative (SOV) vs stem outer green capsule (SOGC).

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Additional file 2:

Analyses of differential gene expression (selected functional groups) between stem inner tissues and stem outer tissues sampled at vegetative and green capsule stages. Lists of differentially expressed genes (selected functional groups only) for i) stem inner vegetative (SIV) vs stem outer vegetative (SOV), ii) stem inner green capsule (SIGC) vs stem outer green capsule (SOGC), iii) stem inner vegetative (SIV) vs stem inner green capsule (SIGC), iv) stem outer vegetative (SOV) vs stem outer green capsule (SOGC). Lists of differentially expressed genes and GO analyses (biological processes) for i) stem inner vegetative (SIV) vs stem outer vegetative (SOV), ii) stem inner green capsule (SIGC) vs stem outer green capsule (SOGC), iii) stem inner vegetative (SIV) vs stem inner green capsule (SIGC), iv) stem outer vegetative (SOV) vs stem outer green capsule (SOGC).

Format: XLS Size: 103KB Download file

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Open Data

Additional file 3:

Differentially expressed genes between Drakkar and Belinka flax cultivars. List of differentially expressed genes between Drakkar and Belinka flax cultivars. Values for up-regulated genes are represented in red and those for down-regulated genes in green colour.

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Additional file 4:

Number of differentially expressed genes between Drakkar and Belinka flax cultivars represented as GO biological process. Drakkar specifically expressed genes are represented as dark columns and those of Belinka as clear columns.

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Additional file 5:

Primer sequences used in qRT-PCR analyses. Sequences of primers used for quantifying target genes by qRT-PCR (Table 1) and Sequences of primers for putative reference genes tested by geNorm (Table 2).

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