Validation of microarray results by qPCR. Genes that showed differential expression from microarray analysis were analyzed in different HAT mutants through qualitative PCR (qPCR). The log2 fold expression ratios from microarray and qPCR experiments for the mutants versus wild type were plotted. qPCR results are indicated by the blue bars, microarray by the purple bars. Strain list: Strain list: Δelp3 (FY 3851), Δgcn5 (FY2943), Δmst2 (FY1890), Δgcn5 Δelp3 (FY3847), Δgcn5 Δmst2(FY3361), Δmst2 Δelp3 (FY3850), Δgcn5 Δelp3 Δmst2 (3854).
Nugent et al. BMC Genomics 2010 11:59 doi:10.1186/1471-2164-11-59