Characterization of mutant phenotypes . A: Δelp3 morphology. Wild-type (FY368) and Δelp3 (FY3851)cells were asynchronously grown at 32°C in YES. Cells were fixed and stained with DAPI. The scale bar represents 10 microns. B: Δelp3 mutants have a growth lag. Representative growth curves for wild type (triangle) (FY368) and Δelp3 (circle) (FY3851) cells in rich media (YES) at 30°C. The x-axis time scale indicates hours after inoculation that stared at a cell concentration of 4 × 105 cells/ml. The y-axis indicates number of cells/ml. C: Comparison of growth rates on YES at different temperatures and with different levels of salt. Cells were grown to exponential phase, serially diluted 5× and compared for growth on rich media after 2 or 3 days at 30°C. and 36°C, or onYES supplemented with indicated salt and grown for 2 to 4 days at 30°C. Strain list: wild-type (FY368), Δelp3(FY3851), Δmst2 (FY1890), Δgcn5 Δmst2(Hu990), Δgcn5 Δelp3 (FY3847), Δmst2 Δelp3 (FY3850), Δgcn5 Δelp3 Δmst2 (3854) D: Comparison of drug sensitivity. Exponentially growing cells were diluted 5-fold on YES plates with the indicated drugs and grown 2-3 days at 32°C. Strain list: wild-type (FY261), Δswi6 (FY1584), Δrad3 (FY1104), Δelp3 (FY 3851), Δgcn5 (FY2943), Δmst2 (FY1890), Δgcn5 Δelp3 (FY3847), Δgcn5 Δmst2(FY3361), Δmst2 Δelp3 (FY3850), Δgcn5 Δelp3 Δmst2 (3854).
Nugent et al. BMC Genomics 2010 11:59 doi:10.1186/1471-2164-11-59