Expression profiling of S. pombe acetyltransferase mutants identifies redundant pathways of gene regulation
- Equal contributors
1 Molecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089-2910, USA
2 Center for Biosciences, Department of Biosciences and Nutrition, Karolinska Institutet, S-141-57 Huddinge, Sweden and School of Life Sciences, Södertörn University, S-141 89 Huddinge, Sweden
3 Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
BMC Genomics 2010, 11:59 doi:10.1186/1471-2164-11-59Published: 22 January 2010
Histone acetyltransferase enzymes (HATs) are implicated in regulation of transcription. HATs from different families may overlap in target and substrate specificity.
We isolated the elp3+ gene encoding the histone acetyltransferase subunit of the Elongator complex in fission yeast and characterized the phenotype of an Δelp3 mutant. We examined genetic interactions between Δelp3 and two other HAT mutants, Δmst2 and Δgcn5 and used whole genome microarray analysis to analyze their effects on gene expression.
Comparison of phenotypes and expression profiles in single, double and triple mutants indicate that these HAT enzymes have overlapping functions. Consistent with this, overlapping specificity in histone H3 acetylation is observed. However, there is no evidence for overlap with another HAT enzyme, encoded by the essential mst1+ gene.