Additional file 6.
Swarm center population contains more dead cells than non-swarming bacteria. Swarming and non-swarming colonies were grown as described in material and methods. For each conditions (swarm center and non-swarming), ten circular agar plugs of 0.75 cm diameter containing swarm center and non-swarming bacteria were extracted from plates and vigorously resuspended in 2 mL of sterile PBS buffer and serially diluted. For CFU count, 100 μL of each serial dilution was plated on TSB agar plates and incubated O/N at 37°C. To determine the dry weight, the remaining bacterial suspensions were placed in pre-weighed aluminum cups and incubated at 65°C for 4 hrs to allow water evaporation. Cups were weighed again to determine total dry weight. All experiments were performed in triplicates.
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Tremblay and Déziel BMC Genomics 2010 11:587 doi:10.1186/1471-2164-11-587