Additional file 9.

Amino acid alignment of substrate recognition sites (SRS) and functional domains for hydroxylation activity (CR1) in plant F3'5'Hs. Amino acid positions crucial for 3' vs. 3'5'-hydroxylation in CR1 and SRS6 are indicated by black arrows; significant amino acid substitutions in grapevine F3'5'Hs are in green background. Relevant amino acid substitutions within domains putatively involved in substrate recognition are highlighted in grapevine F3'5'Hs by blue background when they are unique with respect to all other plant F3'5'Hs or when they are shared exclusively with either monocot F3'5'Hs or other plant F3'Hs, as possible remnants of ancestral transition stages in the evolution of dicot F3'5'Hs.

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Falginella et al. BMC Genomics 2010 11:562   doi:10.1186/1471-2164-11-562