Semi-quantitative Reverse Transcription-PCR (RT-PCR) of candidate genes. Normalized cDNA obtained from reverse transcription of mRNA was used as template for PCR amplifications. Aliquots were taken from PCR reactions at 20, 25 and 30 cycles and analyzed by agarose gel electrophoresis.
Bettencourt et al. BMC Genomics 2010 11:559 doi:10.1186/1471-2164-11-559