Table 2

Effect of protocol optimization and RNase H on qPCR efficiency and linearity

VEGFB

MMP9

TFRC

HAMP

GAPDH

Mean ± SD

P vs -RNase H

P vs original protocol

P vs original protocol/- RNase H


Efficiency (slope)

Original protocol


- RNase H

-1.50

NA

-3.59

-1.15

-1.59

-1.96 ± 1.10

+ RNase H

-3.74

NA

-3.63

-3.13

-3.24

-3.43 ± 0.30

0.003


Optimized protocol

- RNase H

-1.13

-1.24

-2.53

-1.26

-0.65

-1.36 ± 0.70

0.13

+ RNase H

-3.28

-2.83

-3.04

-2.71

-2.54

-2.88 ± 0.29

<0.001

0.16

0.02


Linearity (R2)

Original protocol


- RNase H

0.66

NA

0.97

0.63

0.95

0.80 ± 0.18

+ RNase H

0.99

NA

0.98

0.99

0.97

0.98 ± 0.01

0.16


Optimized protocol


- RNase H

0.99

0.91

0.99

0.72

0.87

0.90 ± 0.11

0.38

+ RNase H

0.98

0.97

0.97

0.99

0.98

0.98 ± 0.01

0.19

0.64

0.16


This table gathers the results obtained from experiments shown in Figure 5 and shows statistical analyses. P values were determined using one-way repeated measures ANOVA and paired t-test. NA: not applicable.

Jeanty et al. BMC Genomics 2010 11:542   doi:10.1186/1471-2164-11-542

Open Data