Table 7

Consistency in detection of differential expression between quantities of starting RNA
reference experiment
test experiment 250 ng 100 ng 50 ng
10 ng 0.62/0.04 0.67/0.04 0.69/0.05
50 ng 0.83/0.07 0.88/0.08
100 ng 0.86/0.06

For the numbers of bead-types for which differential expression between UHRR and Brain was detected we report "X/Y" where X is the proportion of bead-types reported for the reference experiment also reported for the test experiment (e.g. a measure sensitivity), and Y is the proportion of bead-types reported by the test experiment that were not reported by the reference experiment (e.g. FDR). So for example, taking 250 ng as a gold-standard, for this detection measure the 100 ng experiment has 86% sensitivity and an FDR of 0.06.

Lynch et al.

Lynch et al. BMC Genomics 2010 11:540   doi:10.1186/1471-2164-11-540

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