The power to detect differential expression by quantity of starting RNA. Illustrating, for the analysis-group of bead-types, the increased ability to detect large log2-fold changes (for all levels of starting RNA), and how the relationship (between that ability and the size of the log-fold change) varies with the quantity of starting RNA. The empirical log fold change calculated from the 250 ng experiment is depicted on the x-axis, which is divided into 50 bins, each containing 2% of the bead-types (indicated by the vertical lines). On the y-axis are indicated 95% confidence intervals for the proportion of bead-types in each bin for which differential expression will be detected.
Lynch et al. BMC Genomics 2010 11:540 doi:10.1186/1471-2164-11-540