Composite image of graphs of Real Time PCR performed on genes of interest. The real time PCR was performed on OS epithelial regions of LEC, cornea, limbus and conjunctiva comprising of three biological replicates (3 eyes) with four sets of technical replicates A, B, C & D. These were processed in triplicate. The cycle threshold (Ct) value for these samples were averaged and normalised with 18S rRNA Ct values. Significant p values between LEC and other OS regions is shown as (*),*p < 0.05, ***p < 0.001. Data are expressed as means +/- standard errors of the mean (SEM).
Kulkarni et al. BMC Genomics 2010 11:526 doi:10.1186/1471-2164-11-526