Expression profiles of 40 candidate genes. Expression profiles of 40 Immune-related candidate genes (19 from hemocytes SSH library and 21 from fat body SSH library) in host immune tissues. These genes were analyzed by semi-quantitative RT-PCR, using the P. rapae 18 S rRNA as a reference gene. The EST ID of the genes and the PCR primer sequences are given in additional file 4. Gene expression in host hemocytes and fat body cells at 1 h PI, "N" represents the negative control, treatment with Pringle's saline buffer. "I" represents the immune-inducible genes following Sephadex bead injection, and "S" denotes the immune-suppressed following venom treatment. Hemocytes and fat body genes are identified by the red lines.
Fang et al. BMC Genomics 2010 11:484 doi:10.1186/1471-2164-11-484