Table 1

Results of Methylation Specific PCR analysis for five C. gigas genes

Accession #

Best blast hit [Organism]

Undigested

HpaII

MspI

Number of restriction sites

CpG o/e


EW778441

heat shock protein 70

[Crassostrea gigas]

+

+

_

2

0.57


EW777519

heat shock protein 25

[Danio rerio]

+

_

_

3

0.81


EW778166

cytochrome P450

[Haliotis diversicolor]

+

_

_

1

0.85


EW778608

macrophage expressed protein 1-like protein [Crassostrea gigas]

+

_

_

6

1.08


EW778905

14-3-3 protein gamma (Protein kinase C inhibitor protein 1

[Bos taurus])

+

_

_

2

0.92


Results of methylation status of five genes associated with immune response by MSP. PCR was carried out on undigested, HpaII digested, and MspI digested DNA. Presence (+) or absence (-) of PCR product is indicated. Number of CCGG restriction sites in the indicated sequence and CpGo/e ratios are also provided.

Gavery and Roberts BMC Genomics 2010 11:483   doi:10.1186/1471-2164-11-483

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