Table 3

In silico analysis and predicted protein function of 10 contigs with the highest R-value with no putative role assigned by a BLASTX search.

Library Bias

Contig #

Length (bp)

R¹

p-value²

Predicted Protein Function³

Soldier

910

1255

15

6.92^e-11

Antibacterial humoral response; Translation elongation

Alate

545

1346

382

6.39^e-128

Neurotransmitter secretion; Cholesterol absorption

Alate

142

1745

76

1.54^e-25

Cell adhesion; Hydrolase activity

Late Larval

366

478

97

5.45^e-70

Protein biosynthesis; Ligase activity

Late Larval

598

903

49

2.00^e-19

Regulation of transcription; Metabolism

Late Larval

516

393

39

2.04^e-16

Stress Response

Late Larval

533

474

31

7.12^e-13

Cell adhesion

Late Larval

582

1133

26

9.43^e-09

Spermatogenesis; Cell-cell adhesion

Late Larval

560

197

24

3.89^e-09

Ribosomal protein-nucleus import

Late Larval

596

747

14

1.27^e-07

Amino acid metabolism

¹ R-statistic was based on equations in Stekel et al. (2000) and compared relative abundance of each libraries prevalence in contiguous sequences from all available sequences.

² p-values were calculated to establish appropriate significance thresholds to confirm individual library abundance. p-value is in reference to significance of caste bias of singletons in contigs formed using a chi-squared test on singleton representation in each contig.

³ Putative protein functions were predicted using: http://dragon.bio.purdue.edu/pfp webcite.

Steller et al. BMC Genomics 2010 11:463   doi:10.1186/1471-2164-11-463