Table 3

In silico analysis and predicted protein function of 10 contigs with the highest R-value with no putative role assigned by a BLASTX search.

Library Bias

Contig #

Length (bp)

R1

p-value2

Predicted Protein Function3


Soldier

910

1255

15

6.92e-11

Antibacterial humoral response; Translation elongation


Alate

545

1346

382

6.39e-128

Neurotransmitter secretion; Cholesterol absorption


Alate

142

1745

76

1.54e-25

Cell adhesion; Hydrolase activity


Late Larval

366

478

97

5.45e-70

Protein biosynthesis; Ligase activity


Late Larval

598

903

49

2.00e-19

Regulation of transcription; Metabolism


Late Larval

516

393

39

2.04e-16

Stress Response


Late Larval

533

474

31

7.12e-13

Cell adhesion


Late Larval

582

1133

26

9.43e-09

Spermatogenesis; Cell-cell adhesion


Late Larval

560

197

24

3.89e-09

Ribosomal protein-nucleus import


Late Larval

596

747

14

1.27e-07

Amino acid metabolism


1 R-statistic was based on equations in Stekel et al. (2000) and compared relative abundance of each libraries prevalence in contiguous sequences from all available sequences.

2 p-values were calculated to establish appropriate significance thresholds to confirm individual library abundance. p-value is in reference to significance of caste bias of singletons in contigs formed using a chi-squared test on singleton representation in each contig.

3 Putative protein functions were predicted using: http://dragon.bio.purdue.edu/pfp webcite.

Steller et al. BMC Genomics 2010 11:463   doi:10.1186/1471-2164-11-463

Open Data