Table 4 |
||
|
Primers used in the gene mutagenesis in this study |
||
|
Genes |
Primers |
Sequences |
|
|
||
|
ssaN |
ssaN-Km-F |
5'-CTTTGCTATCTCCTTTTACGAGTACAATCGGGCTTCACTGC |
|
GGGCAGCAAGTGATGGCCTAAAGCCACGTTGTGTCTCAA-3'α |
||
|
ssaN-Km-R |
5'-CTTCAGACAGTGTAAAATCGATGAATTCGCGGACTTCTCGT |
|
|
CCACGTTCACCAATTAACAGCGCTGAGGTCTGCCTCGTG-3'α |
||
|
ssaN-F |
5'-CGTTGTTAAATGCGTGGTTG-3' |
|
|
ssaN-R |
5'-CCCATTCCCGTACGTTCTAA-3' |
|
|
ssaR |
ssaR-Km-F |
5'-GCGCTTGTACTTTCCTTATTCATTATGGGGCCGACGCTATT |
|
AGCTGTAAAAGAGCGCTGGAAAGCCACGTTGTGTCTCAA-3'α |
||
|
ssaR-Km-R |
5'-ATTAATATGAGCAAAGAATCAGGTTTTATCTTTCTTTTTAT |
|
|
GTTCTTCAGGCCAGGTTCGGCGCTGAGGTCTGCCTCGTG-3'α |
||
|
ssaR-F |
5'-TATCGCACTGTATGGCCTTG-3' |
|
|
ssaR-R |
5'-ACCGCCTGCCAGTAAAAATA-3' |
|
|
sspH2 |
sspH2-Km-F |
5'-CTTTATGAAGTTTTCCGTCTCACTCAGTCTGTCCAGGAAGA |
|
GGCTGAATGCGTCGGCGTTAAAGCCACGTTGTGTCTCAA-3'α |
||
|
sspH2-Km-R |
5'-ACACTGGTTATTCCTGATAATAATCTGACCAGCCTGCCGGC |
|
|
GCTGCCGCCAGAACTGCGGGCGCTGAGGTTGTGTCTCAA-3'α |
||
|
sspH2-F |
5'-TCATCTTCAGCCAGTTGTGC-3' |
|
|
sspH2-R |
5'-GTAATCGCCGCATTTATCGT-3' |
|
|
|
||
|
α: 80-nucleotide (nt)-long primers including 60 nt homology extensions complementary to the targeted regions of the genes and 20 nt priming sequences (underlined) for the synthesis of the kanamycin resistance cassette gene from E. coli MC4100 ybeW::Km. |
||
|
Ge et al. BMC Genomics 2010 11:456 doi:10.1186/1471-2164-11-456 |
||
