Table 6

List of the used oligonucleotides

FVB/N PrP-/- mice

Name

SEQUENCE (5' -3')

Prnp 5'

CAACCGAGCTGAAGCATTCTG

Prnp 3'

CGACATCAGTCCACATAGTC

scg5 5'

CCTTTATGAGAAAATGAAGGG

scg5 3'

GGACAGATTTCTTTGCCACA

Tg37xNFH-Cre mice

Name

SEQUENCE (5' -3')

Ifitm3 5'

TCAGCATCCTGATGGTTGTT

Ifitm3 3'

TGTTACACCTGCGTGTAGGG

AV451297.1 5'

CCCGAAGCGTTTACTTTGAA

AV451297.1 3'

CCCTCTTAATCATGGCCTCA

Erf1 5'

TCGCTCCACCAACTAAGAAC

Erf1 3'

AAACACGGGAAACCTCACC

Prnp Tg37 5'

GAAGGAGTCCCAGGCCTATT

Prnp Tg37 3'

GCAGGAATGAGACACCACCT

Glrx3 5'

CATAAGCATGGTGTCCAAGG

Glrx3 3'

TGCCTTCTCTGCTTCGTAGA

Riken D17 5'

AAGCCTTCATAGCGAGTGGA

Riken D17 3'

TTCCAGACAAGTGGACCTGA

Bace1 5'

TCGACCACTCGCTATACACG

Bace1 3'

CTCCTTGCAGTCCATCTTGAG

Fgf2 B 5'

AGCGGCTCTACTGCAAGAAC

Fgf2 B 3'

GCCGTCCATCTTCCTTCATA

Atp13a1 5'

CGTGACAAGGGTGAAGATGG

Atp13a1 3'

ATAGTAAGAGAAGGCATTCC

BB217622.2 5'

CCAGTTCCGTCAAAGTACCC

BB217622.2 3'

CATGCAGATCTTCAGGTCCA

β-actin 5'

TGTTACCAACTGGGACGACA

β-actin 3'

GGGGTGTTGAAGGTCTCAAA

The sequences of the oligonucleotides used in the QPCR experiments are listed; including those of the housekeeping gene that was used in the three described analyses. The sets of primers for the Erf1, Glrx3, BB217622.2 and the AV451297.1 loci were designed within a single exon. All the other sets were designed over exon-exon borders.

Chadi et al. BMC Genomics 2010 11:448   doi:10.1186/1471-2164-11-448