Research article

Recovery of the mitochondrial COI barcode region in diverse Hexapoda through tRNA-based primers

Doo-Sang Park¹, Soo-Jung Suh³, Hyun-Woo Oh² and Paul DN Hebert⁴^*

¹ Biological Resource Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Korea

² Industrial Biomaterials Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Korea

³ Youngnam Regional Office, National Plant Quarantine Service, Busan 600-016, Korea

⁴ Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, N1G 2W1, Canada

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BMC Genomics 2010, 11:423 doi:10.1186/1471-2164-11-423

Published: 9 July 2010

Additional file 1:

PCR comparisons. Agarose gels comparing the PCR products generated using both the standard primer set and the tRNA-W primer cocktail.

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Additional file 2:

Initiation codon of the COI. Identification of possible initiation codons for sequences amplified by the tRNA-W cocktail was analyzed.

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Additional file 3:

Scale insect samples used in this study.

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Additional file 4:

Information on samples used in this study.

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